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Programming sites of meiotic crossovers using Spo11 fusion proteins.

Nucleic Acids Res. 2017; 
Sarno R,, Vicq Y,, Uematsu N,, Luka M,, Lapierre C,, Carroll D, Bastianelli G, Serero A,, Nicolas A,.
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摘要

Meiotic recombination shapes the genetic diversity transmitted upon sexual reproduction. However, its non-random distribution along the chromosomes constrains the landscape of potential genetic combinations. For a variety of purposes, it is desirable to expand the natural repertoire by changing the distribution of crossovers in a wide range of eukaryotes. Toward this end, we report the local stimulation of meiotic recombination at a number of chromosomal sites by tethering the natural Spo11 protein to various DNA-binding modules: full-length DNA binding proteins, zinc fingers (ZFs), transcription activator-like effector (TALE) modules, and the CRISPR-Cas9 system. In the yeast Saccharomyces cerevisiae, each stra... More

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