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Octanoylation of early intermediates of mycobacterial methylglucose lipopolysaccharides.

Sci Rep. 2015; 
Maranha A, Moynihan PJ, Miranda V, Correia Lourenço E, Nunes-Costa D, Fraga JS,, José Barbosa Pereira P,, Macedo-Ribeiro S,, Ventura MR, Clarke AJ, Empadinhas N,.
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Custom Vector Construction coli, synthesized (GenScript) and cloned between the Nde I and Hind III restric- tion sites of the expression vector pET30a (Novagen). Get A Quote

摘要

Mycobacteria synthesize unique intracellular methylglucose lipopolysaccharides (MGLP) proposed to modulate fatty acid metabolism. In addition to the partial esterification of glucose or methylglucose units with short-chain fatty acids, octanoate was invariably detected on the MGLP reducing end. We have identified a novel sugar octanoyltransferase (OctT) that efficiently transfers octanoate to glucosylglycerate (GG) and diglucosylglycerate (DGG), the earliest intermediates in MGLP biosynthesis. Enzymatic studies, synthetic chemistry, NMR spectroscopy and mass spectrometry approaches suggest that, in contrast to the prevailing consensus, octanoate is not esterified to the primary hydroxyl group of glycerate but i... More

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