| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Immunogen Biological Dilution Note and Source source Factor acetylated mouse 1: 1000 Sigma, #T6793 α-tubulin GFP GFP HA mouse 1: 1000 Roche, #11814460001 mouse 1: 5000 Abmart, #M20004 rabbit 1: 5000 Roche, #11867423001 histone H3 rabbit 1: 5000 Agrisera, #AS10710 IFT46 rabbit 1: 5000 Prepared by Genscript using the N-terminal 20 amino-acids peptide and purified using the same peptide IFT52 rabbit 1: 250 (Deane et al. | Get A Quote |
Cilia are microtubule-based organelles and perform motile, sensing and signaling functions. The assembly and maintenance of cilia depend on intraflagellar transport (IFT). Besides ciliary localization, most IFT proteins accumulate at basal bodies. However, little is known about the molecular mechanism of basal body targeting of IFT proteins. We first identified the possible basal body-targeting sequence in IFT46 by expressing IFT46 truncation constructs in an ift46-1 mutant. The C-terminal sequence between residues 246-321, termed BBTS3, was sufficient to target YFP to basal bodies in the ift46-1 strain. Interestingly, BBTS3 is also responsible for the ciliary targeting of IFT46. BBTS3::YFP moves bidirectionall... More
