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Effects of Cdh23 single nucleotide substitutions on age-related hearing loss in C57BL/6 and 129S1/Sv mice and comparisons with congenic strains.

Sci Rep. 2017; 
Johnson KR, Tian C, Gagnon LH, Jiang H, Ding D, Salvi R.
Products/Services Used Details Operation
Custom Vector Construction To create the targeting vector, a synthetic gene (Genscript, Piscataway, NJ) consisting of a 300 bp homology arm (Chr10: 60,536,591–60,536,889) and a 320 bp homology arm (Chr10: 60,527,162–60,527,481) and including restriction enzyme sites (HindIII and Xho1, spaced by Kpn1) for retrieval and Not1 for construct release was cloned into the pBlight-TK plasmid33. Get A Quote

摘要

A single nucleotide variant (SNV) of the cadherin 23 gene (Cdh23c.753A), common to many inbred mouse strains, accelerates age-related hearing loss (AHL) and can worsen auditory phenotypes of other mutations. We used homologous recombination in C57BL/6 NJ (B6N) and 129S1/SvImJ (129S1) embryonic stem cells to engineer mouse strains with reciprocal single base pair substitutions (B6-Cdh23c.753A>G and 129S1-Cdh23c.753G>A). We compared ABR thresholds and cochlear pathologies of these SNV mice with those of congenic (B6.129S1-Cdh23Ahl+ and 129S1.B6-Cdh23ahl) and parental (B6N and 129S1) strain mice. Results verified the protective effect of the Cdh23c.753G allele, which prevented high frequency hearing loss in B6 m... More

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