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Dectin-2 Recognizes Mannosylated O-antigens of Human Opportunistic Pathogens and Augments Lipopolysaccharide Activation of Myeloid Cells.

J Biol Chem. 2016; 
Wittmann A, Lamprinaki D, Bowles KM, Katzenellenbogen E, Knirel YA, Whitfield C, Nishimura T, Matsumoto N, Yamamoto K, Iwakura Y, Saijo S, Kawasaki N.
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Custom Vector Construction The DNA fragment encoding extracellular domain of Dectin-2 with the two missense mutations was synthesised (Genscript) and cloned into the pMXs-IRES-EGFP-Ly49A-CD3ζ vector and used as described above. Get A Quote

摘要

LPS consists of a relatively conserved region of lipid A and core oligosaccharide and a highly variable region of O-antigen polysaccharide. Whereas lipid A is known to bind to the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex, the role of the O-antigen remains unclear. Here we report a novel molecular interaction between dendritic cell-associated C-type lectin-2 (Dectin-2) and mannosylated O-antigen found in a human opportunistic pathogen, Hafnia alvei PCM 1223, which has a repeating unit of [-Man-α1,3-Man-α1,2-Man-α1,2-Man-α1,2-Man-α1,3-]. H. alvei LPS induced higher levels of TNFα and IL-10 from mouse bone marrow-derived dendritic cells (BM-DCs), when compared with Salmonell... More

关键词

Toll-like receptor 4 (TLR4); immunology; lectin; lipopolysaccharide (LPS); polysaccharide