| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | Materials and methods Construction of expression plasmids The coding sequence for the C-terminal GIY-YIG domain (amino acid residues 979–1118) of AtMSH1 was synthe- sized (GenScript Japan, Tokyo, Japan) and cloned into the NdeI-BamHI site of the vector pET-15b (Merck, Darm- stadt, Germany) to obtain pET-15b/AtMSH1 C-terminal domain (CTD). | Get A Quote |
In plant organelle genomes, homeologous recombination between heteroallelic positions of repetitive sequences is increased by dysfunction of the gene encoding MutS homolog 1 (MSH1), a plant organelle-specific homolog of bacterial mismatch-binding protein MutS1. The C-terminal region of plant MSH1 contains the GIY-YIG endonuclease motif. The biochemical characteristics of plant MSH1 have not been investigated; accordingly, the molecular mechanism by which plant MSH1 suppresses homeologous recombination is unknown. Here, we characterized the recombinant GIY-YIG domain of Arabidopsis thaliana MSH1, showing that the domain possesses branched DNA-specific DNA-binding activity. Interestingly, the domain exhibited no ... More
