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Recognition of Schistosoma mansoni egg-expressed ovalbumin by T cell receptor transgenic mice.

Exp Parasitol. 2019; 
Tedla MG, Nahar MF, Hagen J, Every AL, Scheerlinck JY.
Products/Services Used Details Operation
Gene Synthesis After the sequence was synthesized commercially by GenScript (NJ, USA), the gene was cloned in to the pGIPZ_CMV_mCherry via SpeI and NotI replacing mCherry to produce pGIPZ Opt-OVA (Fig. Get A Quote

摘要

Schistosoma mansoni eggs can influence immune responses directed at them, and the mechanisms by which this is achieved are being unravelled. Going towards, developing effective tools for the study of how S. mansoni influences naïve T cells, we have developed S. mansoni eggs expressing chicken ovalbumin (OVA), using a lentiviral transduction system. Indeed, such a parasite could be used in conjunction with cells from OT-II transgenic mice as a source of naïve, antigen-specific T cells. The expression of the transgenic protein was confirmed by real-time RT-PCR of OVA-specific mRNA and western blotting using polyclonal antibodies specific for OVA. T cells from OT-II transgenic mice expressing a T cell receptor s... More

关键词

OT II T cells; Ovalbumin; Schistosoma mansoni; Transgenesis