| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | , 2004 N/A Rabbit polyclonal anti-LH4 (GARLH4) This paper N/A Chemicals, Peptides, and Recombinant Proteins Synthetic GABAARa1 peptide: QPSQDELKDNTTVFTC Synthetic GluA2/3 peptide: EGYNVYGIESVKI GenScript N/A GenScript N/A Lauryl Maltose-neopentyl glycol Anatrace Cat#: NG310 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat#: 78446 Tricine Sigma Cat#: T5816 Bis-Tris hydrochloride Sigma Cat#: B6032 Aminocaproic Acid Sigma Cat#: A2504 CBB G-250: SERVA Blue G SERVA Cat#: 35050 NativeMark Unstained Protein Standard Life Technologies Cat#: LC0725 Protein A Sepharose CL4B GE Healthcare Cat#: 17-0780-01 Lipofectamin 2000 Thermo Fisher Scientific Cat#: 11668019 Ficoll 400 Sigma Cat#: F8016 Papain Sigma P4762 Neurobasal Medium GIBCO 21103-045 B-27 Supplement GIBCO 17504-044 GlutaMax GIBCO 35050-061 L-Glutamine GIBCO 25030-081 (Continued on next page) Neuron 93, 1138–1152....0) and eluted by elution buffer containing 1 mM of the a1 antigen peptide QPSQDELKDNTTVFTC synthesized (Genscript), 1% MNG, 40 mM Tris-HCl (pH 8. | Get A Quote |
Ionotropic neurotransmitter receptors mediate fast synaptic transmission by functioning as ligand-gated ion channels. Fast inhibitory transmission in the brain is mediated mostly by ionotropic GABAA receptors (GABAARs), but their essential components for synaptic localization remain unknown. Here, we identify putative auxiliary subunits of GABAARs, which we term GARLHs, consisting of LH4 and LH3 proteins. LH4 forms a stable tripartite complex with GABAARs and neuroligin-2 in the brain. Moreover, LH4 is required for the synaptic localization of GABAARs and inhibitory synaptic transmission in the hippocampus. Our findings propose GARLHs as the first identified auxiliary subunits for anion channels. These findings... More
