| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | The respective primers are: TP53: 5’-CAGTCTACCTCCCGCCATAA-3’ and 5’- CCACAACAAAACACCAGTGC-3’ MMP-2: 5’-CATGTCGCCCCTAAAACAGA-3’ and 5’-CCATCAAACGGGTATCCATC-3’ GAPDH: 5’-GAGTCCACTGGCGTCTTC-3’ and 5’-GGGGTGCTAAGCAGTTGGT-3’ SDF-1: 5’-TAGTCAAGTGCGTCCACGA-3’ and 5’-GGACACACCACAGCACAAAC-3’ α-SMA: 5′-CCGACCGAATGCAGAAGGA-3′ and 5′-ACAGAGTATTTGCGCTCCGAA-3′ TGF-β1: 5’-TGTGTGCTGAAGCCATCGTTG-3’ and 5’-CCGGCTTGTCTGAAAAGGTCA-3’ IL-6: 5’-GACAAAGCCAGAGTCCTTCAGAGA-3’ and 5’-CTAGGTTTGCCGAGTAGATCT-3’ ATR: 5’-GTCATATACACTCCCTTTTCTTTA-3’ and 5’-GTCATATACACTCCCTTTTCTTTA-3’ 34688 ATR-shRNA transfection Cell proliferation ATR-shRNA (KHD1318) expressed in sure silencing shRNA plasmid and the corresponding control plasmid were obtained from GenScript Corporation, and were used to carry out transfection using human dermal fibroblast nucleofector 2000 transfection kit (Invitrogen) following the protocol recommended by the manufacturer. | Get A Quote |
The ATR protein kinase is a master regulator of the cellular responses to DNA damage and replication stresses. Despite these crucial physiological roles, the implication of ATR in human carcinogenesis remains elusive. We have shown here that the ATR level is reduced in most cancer-associated fibroblasts (CAFs) as compared to their adjacent normal counterparts. Importantly, specific ATR knockdown activated breast fibroblasts, and enhanced their paracrine pro-carcinogenic effects via strong increase in the expression/secretion of SDF-1 and IL-6. Furthermore, ATR-deficient fibroblasts enhanced tumor growth and aggressiveness in orthotopic breast tumor xenografts. On the other hand, ectopic expression of ATR suppre... More
