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Protumoral TSP50 Regulates Macrophage Activities and Polarization via Production of TNF-α and IL-1β, and Activation of the NF-κB Signaling Pathway.

PLoS ONE. 2015; 
Yang C, Zhang DM, Song ZB, Hou YQ, Bao YL, Sun LG, Yu CL, Li YX.
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Custom Vector Construction 1/Hygro vector (GenScript Corporation, IA, USA) was used and manipulated as previously described [8].... The sequence of the negative control shRNA (GenScript Corporation, China) was 5 -GACGCTTACCGATTCAGAA-3 , which has no significant homology to mouse or human gene sequences. Get A Quote

摘要

Testes-specific protease 50 (TSP50) is abnormally overexpressed in many kinds of cancers and promotes cell proliferation and migration. However, whether TSP50 can influence the tumor microenvironment, especially the function of immune cells in the microenvironment, remains largely unknown. We demonstrated that exposure to the conditioned medium from TSP50-overexpressing cells, or co-culture with TSP50-overexpressing cells, enhanced the cytokine production and phagocytic activities of macrophages, and induced M2b polarization. Further investigation showed that production of TNF-α and IL-1β was strongly induced by TSP50 in TSP50-overexpressing cells. TSP50-induced TNF-α and IL-1β were main factors that mediat... More

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