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Nidogen-1 is a common target of microRNAs MiR-192/215 in the pathogenesis of Hirschsprung's disease.

J Neurochem. 2015; 
Zhu D,, Xie H,, Li H,, Cai P,, Zhu H,, Xu C,, Chen P,, Sharan A,, Xia Y,, Tang W,.
Products/Services Used Details Operation
Custom Vector Construction Dual-luciferase reporter assay 0 The wild-type and mutated 3 -UTR sequence of NID1 mRNA which were called pGL3-NID1 and pGL3-NID1-mut were inserted into the KpnI and SacI sites of pGL3 promoter vector (Genscript, Nanjing, China) in Dual-luciferase reporter assay. Get A Quote

摘要

Recent studies have emphasized the important role of microRNA (miRNA) clusters and common target genes in disease progression. Despite the known involvement of the miR-192/215 family in many human diseases, its biological role in Hirschsprung disease (HSCR) remains undefined. In this study, we explored the role of the miR-192/215 family in the pathogenesis of HSCR. Quantitative real-time PCR and western blotting measured relative expression levels of miRNAs, mRNAs, and proteins in 80 HSCR patients and 77 normal colon tissues. Targets were evaluated by dual-luciferase reporter assays, and the functional effects of miR-192/215 on human 293T and SH-SY5Y cells were detected by the Transwell assay, CCK8 assay and f... More

关键词

3′-UTR; HSCR; gene regulation; microRNA; neural crest cell; neural development