| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The flanking ITRs within the respective transposon vectors were synthesized (GenScript; NY, USA) originating from the T c - l i k e e l em e n t (GenBank accession no....1) was obtained by gene synthesis (GenScript, NY, USA) flanked by the restriction side motifs of EcoRV and BstXI and inserted accordingly into IhW and SB-IhW, respectively, to obtain the envelope constructs. | Get A Quote |
Viral vector particles derived from murine leukemia virus (MLV) mediate highly efficient stable gene transfer used in gene therapeutic approaches and in the generation of transgenic cell lines. However, the establishment of stable viral packaging cells (VPCs) is a time-consuming challenge. To overcome this limitation, we successfully generated novel Sleeping Beauty-derived transposon vectors entailing envelope and packaging expression cassettes as well as a transfer vector. Upon multiplexed transposition in human cells, VPC bulk populations yielding titers of over 1 × 106 transduction-competent vectors were established within three weeks. In contrast, conventional plasmid-based establishment of VPCs, condu... More
