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| Plasmid DNA Preparation | htm Various synthetic peptides (Table 1, this paper) Genscript N/A Morpheus screen condition C10 Critical Commercial Assays UDP-Glo glycosyltransferase kit Deposited Data Crystal structure, Apo XT1 Crystal structure, XT1 + peptide 1 Crystal structure, XT1 + peptide 2 + UDP-xylose Crystal structure, XT1 + peptide 3 Crystal structure, XT1 + peptide 4 Crystal structure, XT1 +peptide 5 Crystal structure, XT1 +peptide 6 Crystal structure, XT1 +peptide 7 Crystal structure, XT1 +peptide 8 Experimental Models: Cell Lines HEK293-F Oligonucleotides PCR amplification primers for XT1 construct: 5’-CGGAATTCGCTAGCCGATGTGTCCAGACCGCCT 5’-AGGATCCGCGGCCGCCCTGAGCCGGCCATCAGG Recombinant DNA Human XT1 cDNA Plasmid pCEP-Pu-N-His-TEV Software and Algorithms XIA2 XDS DIALS AIMLESS CTRUNCATE POINTLESS PHASER PHENIX COOT Molecular Dimensions MDSR-47-C10 Promega Cat# V6961 This paper This paper This paper This paper This paper This paper This paper This paper This paper PDB: 6FOA PDB: 6EJ8 PDB: 6EJ7 PDB: 6EJ9 PDB: 6EJA PDB: 6EJB PDB: 6EJC PDB: 6EJD PDB: 6EJE Thermo Fisher R79007 Life Technologies N/A Dharmacon BC045778 Clone ID: 4791553 Pulido et al.... Peptides (Genscript) and UDP-xylose (Carbosource) were soaked into the crystals either by sequential addition of ligand to the crystallisation drop (up to final concentrations of peptide and UDP-xylose of 0. | Get A Quote |
Proteoglycans (PGs) are essential components of the animal extracellular matrix and are required for cell adhesion, migration, signaling, and immune function. PGs are composed of a core protein and long glycosaminoglycan (GAG) chains, which often specify PG function. GAG biosynthesis is initiated by peptide O-xylosyltransferases, which transfer xylose onto selected serine residues in the core proteins. We have determined crystal structures of human xylosyltransferase 1 (XT1) in complex with the sugar donor, UDP-xylose, and various acceptor peptides. The structures reveal unique active-site features that, in conjunction with functional experiments, explain the substrate specificity of XT1. A constriction within ... More
