| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | 0 all-in-one vector (GenScript, Piscataway, NJ, USA) encoding Cas9 and a gRNA sequence (5’ CTTCTTCTCTGGCGCGTTC 3’) targeting a region within the murine Foxc2 gene that encodes a portion of the N-terminal forkhead-binding domain of FOXC2.... Sanger sequencing was then performed on the purified PCR products using GenScript’s DNA Sequencing Service in order to assess Foxc2 gene editing. | Get A Quote |
The FOXC2 transcription factor promotes the progression of several cancer types, but has not been investigated in the context of melanoma cells. To study FOXC2's influence on melanoma progression, we generated a FOXC2-deficient murine melanoma cell line and evaluated The Cancer Genome Atlas (TCGA) patient datasets.,We compared tumor growth kinetics and RNA-seq/qRT-PCR gene expression profiles from wild-type versus FOXC2-deficient murine melanomas. We also performed Kaplan-Meier survival analysis of TCGA data to assess the influence of FOXC2 gene expression on melanoma patients' response to chemotherapy and immunotherapy.,FOXC2 promotes melanoma progression and regulates the expression of genes associated with m... More
