| Products/Services Used | Details | Operation |
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| Molecular Biology Reagents | 3-(4,5 dimethylthiazol-2yl)2 ,5diphenyltetrazolium bromide (MTT) and propodium iodide were obtained from Sigma- Aldrich (SaintLouis,USA), Ribonuclease A (iNtRON Biotechnology, Seoul, Korea), Paclitaxel was obtained from Stragen (Switzerland), Caspase-3 coloremetric assay kit was purchased from GenScript (NJ, USA) and RT-PCR reagents were provided by iNtRON Biotechnology, Seoul, Korea.... Detection of apoptosis and determination of cell cycle phase The level of an apoptotic marker, cleaved caspase-3 (ac t ive form) was measured in ce l l lysa tes us ing a colorimetric assay kit (GenScript, NJ, USA) according to the manufacturer ’s instructions. | Get A Quote |
Galectin-3 (Gal-3) is a carbohydrate-binding protein which is thought to be involved in cancer progression but its contribution to epithelial ovarian cancer (EOC) remains unclear. The present study sought to determine the role of Gal-3 in chemoresistance of the human SKOV-3 ovarian cancer cell line to paclitaxel (PTX) using recombinant human Gal-3 (rhGal-3) and PectaSol-C modified citrus pectin (Pect-MCP) as a specific Gal-3 competitive inhibitor. Our results showed 41% increased cell proliferation, 36% decreased caspase-3 activity and 33.6% increased substrate-dependent adhesion in the presence of rhGal-3 compared to the control case (p<0.001). Treatment of cells with a non-effective dose of PTX (100nM) and 0.... More
