Cas9-PF, an early flowering and visual selection marker system, enhances the frequency of editing event occurrence and expedites the isolation of genome-edited and transgene-free plants.
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Gene Synthesis> |
A PF cassette containing tandem PAP1 and NtFT expression elements was designed and synthesized by GenScript (Nanjing, China). |
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CRISPR-Cas9; early flowering; genome editing; tobacco; visual selectable marker