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Custom Vector Construction> | … The structural similarity between designs and known helical repeat proteins was assessed by TM-align 36 on RepeatsDB 37 representative structures. Protein expression and characterization. Genes were synthesized and cloned in vector pET21 by GenScript (Piscataway) … | Get A Quote |
A central question in protein evolution is the extent to which naturally occurring proteins sample the space of folded structures accessible to the polypeptide chain. Repeat proteins composed of multiple tandem copies of a modular structure unit are widespread in nature and have critical roles in molecular recognition, signalling, and other essential biological processes. Naturally occurring repeat proteins have been re-engineered for molecular recognition and modular scaffolding applications. Here we use computational protein design to investigate the space of folded structures that can be generated by tandem repeating a simple helix-loop-helix-loop structural motif. Eighty-three designs with sequences unrelat... More