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Inhibition of the thyroid hormonogenic H2O2 production by Duox/DuoxA in zebrafish reveals VAS2870 as a new goitrogenic compound.

Mol Cell Endocrinol. 2020;

Giusti N1, Gillotay P2, Trubiroha A3, Opitz R4, Dumont JE5, Costagliola S6, De Deken X7.

Products/Services Used	Details	Operation
Plasmid DNA Preparation	… The complete coding sequence (990 bp) for the zebrafish Duoxa (NCBI,<br> XM_002666907), chemically synthetized in the pJet1.2 vector (<b>GenScript</b>), was<br> subcloned into the pcDNA3 plasmid using the XhoI and XbaI restriction sites …	Get A Quote

摘要

Thyroid hormone (TH) synthesis requires extracellular hydrogen peroxide generated by the NADPH oxidases, DUOX1 and DUOX2, with maturation factors, DUOXA1 and DUOXA2. In zebrafish, only one duox and one duoxa gene are present. Using a thyroid-specific reporter line, we investigated the role of Duox and Duoxa for TH biosynthesis in zebrafish larvae. Analysis of several zebrafish duox and duoxa mutant models consistently recovered hypothyroid phenotypes with hyperplastic goiter caused by impaired TH synthesis. Mutant larvae developed enlarged thyroids and showed increased expression of the EGFP reporter and thyroid functional markers including wild-type and mutated duox and duoxa transcripts. Treatment of zebrafis... More