Products/Services Used | Details | Operation |
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Gene Synthesis> | The synthetic gene fragments were obtained from GenScript and IDT.... The selected iNKT TCR a and b pair cDNAs were then synthesized as a single bicistonic gene, with codon optimization and an F2A sequence linking the TCRa and TCRb cDNAs to enable their co- expression (GenScript). | Get A Quote |
Invariant natural killer T (iNKT) cells are potent immune cells for targeting cancer; however, their clinical application has been hindered by their low numbers in cancer patients. Here, we developed a proof-of-concept for hematopoietic stem cell-engineered iNKT (HSC-iNKT) cell therapy with the potential to provide therapeutic levels of iNKT cells for a patient's lifetime. Using a human HSC engrafted mouse model and a human iNKT TCR gene engineering approach, we demonstrated the efficient and long-term generation of HSC-iNKT cells in vivo. These HSC-iNKT cells closely resembled endogenous human iNKT cells, could deploy multiple mechanisms to attack tumor cells, and effectively suppressed tumor growth... More