至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Development of Hematopoietic Stem Cell-Engineered Invariant Natural Killer T Cell Therapy for Cancer.

Cell Stem Cell. 2019; 
Zhu Yanni,Smith Drake J,Zhou Yang,Li Yan-Ruide,Yu Jiaji,Lee Derek,Wang Yu-Chen,Di Biase Stefano,Wang Xi,Hardoy Christian,Ku Josh,Tsao Tasha,Lin Levina J,Pham Alexander T,Moon Heesung,McLaughlin Jami,Cheng Donghui,Hollis Roger P,Campo-Fernandez Beatriz,Urbinati Fabrizia,Wei Liu,Pang Larry,Rezek Valerie,Berent-Maoz Beata,Macabali Mignonette H,Gjertson David,Wang Xiaoyan,Galic Zoran,Kitchen Scott G,An Dong Sung,Hu-Lieskovan Siwen,Kaplan-Lefko Paula J,De Oliveira Satiro N,Seet Christopher S,Larson Sarah M,Forman Stephen J,Heath James R,Zack Jerome A,Crooks Gay M,Radu Caius G,Ribas Antoni,Kohn Donald B,Witte Owen N,Yang
Products/Services Used Details Operation
Gene Synthesis The synthetic gene fragments were obtained from GenScript and IDT.... The selected iNKT TCR a and b pair cDNAs were then synthesized as a single bicistonic gene, with codon optimization and an F2A sequence linking the TCRa and TCRb cDNAs to enable their co- expression (GenScript). Get A Quote

摘要

Invariant natural killer T (iNKT) cells are potent immune cells for targeting cancer; however, their clinical application has been hindered by their low numbers in cancer patients. Here, we developed a proof-of-concept for hematopoietic stem cell-engineered iNKT (HSC-iNKT) cell therapy with the potential to provide therapeutic levels of iNKT cells for a patient's lifetime. Using a human HSC engrafted mouse model and a human iNKT TCR gene engineering approach, we demonstrated the efficient and long-term generation of HSC-iNKT cells in vivo. These HSC-iNKT cells closely resembled endogenous human iNKT cells, could deploy multiple mechanisms to attack tumor cells, and effectively suppressed tumor growth... More

关键词

HSC,HSC transfer,HSCT,T cell receptor,TCR,cancer immunotherapy,cell therapy,gene therapy,hematopoietic stem cell,iNKT,invariant natural killer T cell,preclinical development,sr39TK suicide gene,stem cell the