| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Tuftsin binding assay To observe tuftsin binding to the cell surface in the presence or absence of Nrp1 inhibitor EG00229, primary microglia cells were exposed to varying concentrations of inhibitor at 37°C for 1 h, and then treated with 100 lg/mL of biotinylated tuftsin (Genscript, Piscataway, NJ, USA) for 30 min at 21°C to minimize internali- zation of the peptide. | Get A Quote |
Tuftsin (Thr-Lys-Pro-Arg) is a natural immunomodulating peptide found to stimulate phagocytosis in macrophages/microglia. Tuftsin binds to the receptor neuropilin-1 (Nrp1) on the surface of cells. Nrp1 is a single-pass transmembrane protein， but its intracellular C-terminal domain is too small to signal independently. Instead， it associates with a variety of coreceptors. Despite its long history， the pathway through which tuftsin signals has not been described. To investigate this question， we employed various inhibitors to Nrp1's coreceptors to determine which route is responsible for tuftsin signaling. We use the inhibitor EG00229， which prevents tuftsin binding to Nrp1 on the surface of microglia a... More
