| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | For 14-day cultures, PBMCs (1 × 106 cells/ml) were incubated with 10 µM peptides (GenScript) and supplemented with 25 U/ml rIL2 (R&D Systems).... Stock peptides (Genscript, USA) at 10 mg/ml DMSO were added to HLA and β2 m in refolding buffer for 72 h, followed by dialy- sis against 10 mM Tris-HCl (pH 8. | Get A Quote |
Host CD8 T cell response to viral infections involves recognition of 8-10-mer peptides presented by MHC-I molecules. However， proteasomes generate predominantly 2-7-mer peptides， but the role of these peptides is largely unknown. Here， we show that single short peptides of <8-mer from Latent Membrane Protein 2 (LMP2) of Epstein Barr Virus (EBV) can bind HLA-A*11:01 and stimulate CD8 cells. Surprisingly， two peptide fragments between 4-7-mer derived from LMP2 were able to complement each other， forming combination epitopes that can stimulate specific CD8 T cell responses. Moreover， peptides from self-antigens can complement non-self peptides within the HLA binding cleft， forming neoepitopes. Solved... More
