| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The first-strand cDNA was synthesized from 3 μg of total RNA Candidate transcriptomic genes from the RNA-seq were selected and validated by performed in 20 μl reactions by an Applied Biosystem 7500 real-time PCR system commercially (Genscript Bioscience, Nanjing, China) with the product size ranged Accepted M anuscript X33 were germinated on 1/2 MS medium (1/2 macro salts plus 15 g/L glucose, pH were treated with different concentrations of 1-aminocyclopropane-1-carboxylic acid (ACC, Sigma). | Get A Quote |
Abscission is a process that allows plants to shed tissues or organs via cell separation， and occurs throughout the life cycle. Removal of leaves through the use of chemical defoliants is very important for mechanical harvesting of cotton (Gossypium hirsutum). However， our knowledge of the molecular mechanisms of the defoliation response involved is limited. In this study， RNA-seq was conducted in order to profile the differentially expressed genes (DEGs) between cultivars X50 (sensitive to chemical defoliants) and X33 (relatively insensitive) at different time points after treatment with thidiazuron and ethephon (TE). A total of 2434 DEGs were identified between the two cultivars across the different tim... More
