Outer membrane porins， as the major components of Gram-negative bacterial membrane proteins， have been proven to be involved in interactions with the host immune system and potent protective antigen candidates against bacterial infection in fish. Outer membrane porin F (OmpF) is one of the major porins of ()， the causative agent of enteric red mouth disease of salmonid and non-salmonid fish. In the present study， the molecular characterization and phylogenetic analysis of OmpF gene was studied， heterogenous expression， immunogenicity and protective immunity of OmpF were systemically evaluated as a subunit vaccine for channel catfish against infection. The results showed that OmpF gene was highly c... More
Outer membrane porins， as the major components of Gram-negative bacterial membrane proteins， have been proven to be involved in interactions with the host immune system and potent protective antigen candidates against bacterial infection in fish. Outer membrane porin F (OmpF) is one of the major porins of ()， the causative agent of enteric red mouth disease of salmonid and non-salmonid fish. In the present study， the molecular characterization and phylogenetic analysis of OmpF gene was studied， heterogenous expression， immunogenicity and protective immunity of OmpF were systemically evaluated as a subunit vaccine for channel catfish against infection. The results showed that OmpF gene was highly conserved among 15 known species based on the analysis of conserved motifs， sequences alignment and phylogenetic tree， and was subjected to negative/purifying selection with global dN/dS ratios value of 0.649 throughout the evolution. Besides， OmpF was also identified to have immunogenicity by western blotting and was verified to be located on the surface of using cell surface staining and indirect immunofluorescence assays. Moreover， recombinant OmpF (rtOmpF) as a subunit vaccine was injected with commercial adjuvant ISA763， significantly enhanced the immune response by increasing serum antibody levels， lysozyme activity， complement C3 activity， total protein content， SOD activity， immune-related genes expression in the head kidney and spleen， and survival percent of channel catfish against infection. Thus， our present results not only enriched the information of molecular characterization and phylogenetics of OmpF， but also demonstrated that OmpF holds promise to be used as a potential antigen against infection in fish.
