至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The Cacna1h mutation in the GAERS model of absence epilepsy enhances T-type Ca currents by altering calnexin-dependent trafficking of Ca3.2 channels.

Sci Rep. 2017; 
Proft Juliane,Rzhepetskyy Yuriy,Lazniewska Joanna,Zhang Fang-Xiong,Cain Stuart M,Snutch Terrance P,Zamponi Gerald W,Weiss Nor
Products/Services Used Details Operation
Custom Vector Construction synthetized in vitro (GenScript) and subcloned into the XhoI/ HindIII sites of pEGFP-C1 vector. To generate Get A Quote

摘要

Low-voltage-activated T-type calcium channels are essential contributors to the functioning of thalamocortical neurons by supporting burst-firing mode of action potentials. Enhanced T-type calcium conductance has been reported in the Genetic Absence Epilepsy Rat from Strasbourg (GAERS) and proposed to be causally related to the overall development of absence seizure activity. Here, we show that calnexin, an endoplasmic reticulum integral membrane protein, interacts with the III-IV linker region of the Ca3.2 channel to modulate the sorting of the channel to the cell surface. We demonstrate that the GAERS missense mutation located in the Ca3.2 III-IV linker alters the Ca3.2/calnexin interaction, resulting... More

关键词