Products/Services Used | Details | Operation |
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Custom Vector Construction> | synthetized in vitro (GenScript) and subcloned into the XhoI/ HindIII sites of pEGFP-C1 vector. To generate | Get A Quote |
Low-voltage-activated T-type calcium channels are essential contributors to the functioning of thalamocortical neurons by supporting burst-firing mode of action potentials. Enhanced T-type calcium conductance has been reported in the Genetic Absence Epilepsy Rat from Strasbourg (GAERS) and proposed to be causally related to the overall development of absence seizure activity. Here, we show that calnexin, an endoplasmic reticulum integral membrane protein, interacts with the III-IV linker region of the Ca3.2 channel to modulate the sorting of the channel to the cell surface. We demonstrate that the GAERS missense mutation located in the Ca3.2 III-IV linker alters the Ca3.2/calnexin interaction, resulting... More