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Constitutive expression and cell-surface display of a bacterial β-mannanase in Lactobacillus plantarum.

Microb Cell Fact. 2019-04;

NguyenHoang-Minh，PhamMai-Lan，StelzerElena Maria，PlattnerEsther，GrabherrReingard，MathiesenGeir，PeterbauerClemens K，HaltrichDietmar，NguyenTh

Products/Services Used	Details	Operation
Gene Synthesis	The plasmids pSIP_1261ManB [22] containing the mannanase gene (manB) from B. licheniformis DSM13 (ATCC 14580) and pUC57 (provided by GenScript) containing the synthetic constitutive promoter pgm from L. acidophilus NCFM were used as the templates for the amplification of ManB and the Pgm promoter. All plasmids used in this study are listed in Table 1.	Get A Quote

摘要

Lactic acid bacteria (LAB) are important microorganisms in the food and beverage industry. Due to their food-grade status and probiotic characteristics， several LAB are considered as safe and effective cell-factories for food-application purposes. In this present study， we aimed at constitutive expression of a mannanase from Bacillus licheniformis DSM13， which was subsequently displayed on the cell surface of Lactobacillus plantarum WCFS1， for use as whole-cell biocatalyst in oligosaccharide production.