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Detection of Langat virus by TaqMan real-time one-step qRT-PCR method.

Sci Rep. 2015; 
Muhd RadziSiti Fatimah,RückertClaudia,SamSing-Sin,TeohBoon-Teong,JeePui-Fong,PhoonWai-Hong,AbubakarSazaly,ZandiKe
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Custom Vector Construction … The sequences for all viral E genes used in this study were retrieved from GenBank (Table 2). The E genes of the five viruses were synthesized and cloned in pET-51b(+) vector by Genscript company (Genscript, Piscataway, NJ) followed by sequencing to show the accuracy of … Get A Quote

摘要

Langat virus (LGTV), one of the members of the tick-borne encephalitis virus (TBEV) complex, was firstly isolated from Ixodes granulatus ticks in Malaysia. However, the prevalence of LGTV in ticks in the region remains unknown. Surveillance for LGTV is therefore important and thus a tool for specific detection of LGTV is needed. In the present study, we developed a real-time quantitative reverse-transcription-polymerase chain reaction (qRT-PCR) for rapid detection of LGTV. Our findings showed that the developed qRT-PCR could detect LGTV at a titre as low as 0.1 FFU/ml. The detection limit of the qRT-PCR assay at 95% probability was 0.28 FFU/ml as determined by probit analysis (p ≤ 0.05). Besides... More

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