| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … mmol/L NaCl, pH 8.0. After ultrasonic cell disruption, the suspension was centrifuged at 8000 £g for 15 min. The supernatant was bound to high-affinity Ni-charged resin beads (GenScript, USA). The beads were washed in 50 … | Get A Quote |
It has been demonstrated that the lectin from (BanLec-1) could inhibit the cellular entry of human immunodeficiency virus (HIV). In order to evaluate its effects on tobacco mosaic virus (TMV)， the gene was cloned and transformed into and tobacco， respectively. Recombinant BanLec-1 showed metal ions dependence， and higher thermal and pH stability. Overexpression of in tobacco resulted in decreased leaf size， and higher resistance to TMV infection， which includes reduced TMV cellular entry， more stable chlorophyll contents， and enhanced antioxidant enzymes. BanLec-1 was found to bind directly to the TMV capsid protein ， and to inhibit TMV infection in a dose-dependent manner. In contrast to li... More
