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BmAly is an important factor in meiotic progression and spermatid differentiation in Bombyx mori (Lepidoptera: Bombycidae).

J. Insect Sci.. 2014; 
ZhangPengjie,ZhongJinfeng,CaoGuangli,XueRenyu,GongChengl
Products/Services Used Details Operation
Custom Vector Construction The Bmaly PCR product was ligated into vector pMD19-T, and the fragment of Bmaly was excised out with BamHI/ XhoI and ligated with the expression vector pGS-21a(þ) (GenScript, Nanjing, China) to generate the recombinant plasmid pGS-21a(þ)- Bmaly Get A Quote

摘要

The Drosophila melanogaster "always early" gene (Dmaly), which is required for G2/M cell-cycle control and spermatid differentiation, is one of the meiotic arrest genes. To study the Bombyx mori aly gene (Bmaly), the cDNA of Bmaly was cloned and sequenced, and the results showed that the open reading frame of Bmaly is 1,713 bp in length, encoding 570 amino acid residues, in which a domain in an Rb-related pathway was found. Phylogenetic analysis based on the amino acid sequence of conserved regions showed that Aly from different insects gathered together, except for DmAly and Culex quinquefasciatus Aly, which were not clustered to a subgroup according to insect order. The Bmaly gene was insert... More

关键词

Bombyx mori,always early gene,meiosis,spermatogen