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Detection of IFN-γ Secretion by T Cells Collected Before and After Successful Treatment of Early Lyme Disease.

Clin. Infect. Dis.. 2016; 
CallisterSteven M,JobeDean A,Stuparic-StancicAleksandra,MiyamasuMisato,BoyleJeff,DattwylerRaymond J,ArnaboldiPa
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Peptide Synthesis The C6 peptide (CMKKDDQIAAAMVLRGMAKDGQFALK) was synthesized (>95% purity) with biotinylated amine terminal ends (GenScript, Inc, Piscataway, New Jersey), and the ELISA was performed as described previously [21]. Get A Quote

摘要

Current serodiagnostics for Lyme disease lack sensitivity during early disease, and cannot determine treatment response. We evaluated an assay based on QuantiFERON technology utilizing peptide antigens derived from Borrelia burgdorferi to stimulate interferon-gamma (IFN-γ) release as an alternative to serodiagnosis for the laboratory detection of Lyme disease.

关键词

Borrelia burgdorferi,IFN-γ,Lyme disease,T cell,cytokine release a