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Genetic engineering of Yarrowia lipolytica for enhanced production of trans-10， cis-12 conjugated linoleic acid.

Microb. Cell Fact.. 2013; 
ZhangBaixi，ChenHaiqin，LiMin，GuZhennan，SongYuanda，RatledgeColin，ChenYong Q，ZhangHao，Che
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Gene Synthesis The four tandem UAS1B sequence fragment, UAS1B4 was synthesized (Genscript, Nanjing, China), flanked with Cla I-Hin dIII-Spe I sites on the 5′-end and Mlu I-Xba I sites on the 3′-end. The synthetic UAS1B4 sequence was inserted into pUC57 to form pUC-UAS1B4. Get A Quote

摘要

Conjugated linoleic acid (CLA) has been extensively studied for decades because of its health benefits including cancer prevention， anti-atherogenic and anti-obesity effects， and modulation of the immune system. We previously described the production of trans-10， cis-12 CLA in Yarrowia lipolytica by expressing the gene coding for linoleic acid isomerase from Propionibacterium acnes (pai). However the stable strain produced CLA at about 0.08% of dry cell weight (DCW)， a level of production which was not high enough for practical applications. The goal of the present study was to enhance production of CLA by genetic engineering of Y. lipolytica strains.

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