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Consistent mutational paths predict eukaryotic thermostability.

BMC Evol. Biol.. 2013;

van NoortVera，BradatschBettina，ArumugamManimozhiyan，AmlacherStefan，BangeGert，CreeveyChris，FalkSebastian，MendeDaniel R，SinningIrmgard，HurtEd，Bork

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Custom Vector Construction	ORFs for cgARX1, ctARX1, ctarx1-destab and ctarx1-nondestab were synthesized and sequenced by Eurofins MWG Operon (Ebersberg, Germany) or GenScript (Piscataway, NJ, USA) and subcloned into pET-24a(+) vector. Proteins were expressed in E. coli BL21 (DE3) grown in LB-medium at 37°C under vigorous shaking.	Get A Quote

摘要

Proteomes of thermophilic prokaryotes have been instrumental in structural biology and successfully exploited in biotechnology， however many proteins required for eukaryotic cell function are absent from bacteria or archaea. With Chaetomium thermophilum， Thielavia terrestris and Thielavia heterothallica three genome sequences of thermophilic eukaryotes have been published.