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Poly-LacNAc as an age-specific ligand for rotavirus P[11] in neonates and infants.

PLoS ONE. 2013; 
LiuYang,HuangPengwei,JiangBaoming,TanMing,MorrowArdythe L,Jia
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Custom Vector Construction The VP8* sequences (amino acids 46-231) of a human RV P[11] strain N155 (GenBank accession: EU200796) were synthesized by GenScrip (GenScript USA Inc., Piscataway, NJ), and then sub-cloned into the expression vector PGEX-4T-1(GE Healthcare Life Sciences, Piscataway, NJ) between the BamH I and Sal I sites. Get A Quote

摘要

Rotavirus (RV) P[11] is an unique genotype that infects neonates. The mechanism of such age-specific host restriction remains unknown. In this study, we explored host mucosal glycans as a potential age-specific factor for attachment of P[11] RVs. Using in vitro binding assays, we demonstrated that VP8* of a P[11] RV (N155) could bind saliva of infants (60.3%, N = 151) but not of adults (0%, N = 48), with a significantly negative correlation between binding of VP8* and ages of infants (P<0.01). Recognition to the infant saliva did not correlate with the ABO, secretor and Lewis histo-blood group antigens (HBGAs) but with the binding of the lectin Lycopersicon esculentum (LEA) that is known to ... More

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