| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Fixed clathrin cages resuspended in 100 mM potassium phosphate, pH 7.0, were incubated with TP1 peptide (1 mg/mL final concentration, GenScript) for 15 min. resulting pellet was rinsed 4times with water andthen resuspended in water. Fixed clathrin cages resuspended in 2 mM cobalt chloride, pH 6.0, were incubated with either TP1, TP2, or no peptide (1 mg/mL final concentration, GenScript) for 1 h，Fixed clathrin cages resuspended in 100 mM potassium phosphate, pH 7.0, were incubated with TP3 peptide (1 mg/mL final concentration, GenScript) for 15 min | Get A Quote |
Natural systems often utilize a single protein to perform multiple functions. Control over functional specificity is achieved through interactions with other proteins at well-defined epitope binding sites to form a variety of functional coassemblies. Inspired by the biological use of epitope recognition to perform diverse yet specific functions， we present a Template Engineering Through Epitope Recognition (TEThER) strategy that takes advantage of noncovalent， molecular recognition to achieve functional versatility from a single protein template. Engineered TEThER peptides span the biologic-inorganic interface and serve as molecular bridges between epitope binding sites on protein templates and selected ino... More
