Previous studies have shown that foamy viral (FV) vectors are a promising alternative to gammaretroviral and lentiviral vectors and also that insulators can improve FV vector safety. However， in a previous analysis of insulator effects on FV vector safety， strong viral promoters were used to elicit genotoxic events. In the present study， we developed and analyzed the efficacy and safety of a high-titer， clinically relevant FV vector driven by the housekeeping promoter elongation factor-1α and insulated with an enhancer blocking A1 insulator (FV-EGW-A1).
Previous studies have shown that foamy viral (FV) vectors are a promising alternative to gammaretroviral and lentiviral vectors and also that insulators can improve FV vector safety. However， in a previous analysis of insulator effects on FV vector safety， strong viral promoters were used to elicit genotoxic events. In the present study， we developed and analyzed the efficacy and safety of a high-titer， clinically relevant FV vector driven by the housekeeping promoter elongation factor-1α and insulated with an enhancer blocking A1 insulator (FV-EGW-A1).
