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Custom Vector Construction> | … The initial denaturation step was performed at 94°C for 4 min. An A-overhang was attached to the PCR products using Taq DNA polymerase (GenScript Corporation, Piscataway, NJ) and the PCR products were cloned into the pCR 2.1 vector (Invitrogen, Carlsbad, CA). The … | Get A Quote |