| Products/Services Used | Details | Operation |
|---|---|---|
| Plasmid DNA Preparation | … A colony of positive cells was selected on LB agar plates containing ampicillin (100 μg/ml) and screened by direct colony PCR with IL-22 primer (Table 1). The extracted plasmid designated pET-32a-IL-22 was subjected to DNA sequencing by Genscript, Nanjing, China … | Get A Quote |
Pigs suffer enteritis induced by pathogenic bacteria infection and toxins in the moldy feed， which cause intestinal epithelial damage and diarrhea through the whole breeding cycle. Interleukin-22 (IL-22) plays a critical role in maintaining intestinal mucosal barrier function through repairing intestinal epithelial damage. However， little was known about the effects of IL-22 against apoptosis caused by toxins and infection of intestinal pathogens in the intestinal epithelium， especially in pigs. In this study， we had successfully used prokaryotic expression system to produce recombinant porcine interleukin-22. Meanwhile， purified rIL-22 could activate STAT3 signal pathway and have been demonstrated to... More
