| Products/Services Used | Details | Operation |
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| Plasmid DNA Preparation | Briefly, MDA-MB-231 cells were transfected with the empty expression vector or the ERα36-specific shRNA expression vector pRNAT-U6.1/Neo plasmid containing the shRNA against ERα36 (GenScript, USA) with Lipofectamine 3000 according to the manufacturer's instruction. | Get A Quote |
Anthocyanins have been shown to inhibit the growth and metastatic potential of breast cancer (BC) cells. However， the effects of individual anthocyanins on triple-negative breast cancer (TNBC) have not yet been studied. In this study， we found that cyanidin-3-o-glucoside (Cy-3-glu) preferentially promotes the apoptosis of TNBC cells， which co-express the estrogen receptor alpha 36 (ERα36) and the epidermal growth factor receptor (EGFR). We demonstrated that Cy-3-glu directly binds to the ligand-binding domain (LBD) of ERα36， inhibits EGFR/AKT signaling， and promotes EGFR degradation. We also confirmed the therapeutic efficacy of Cy-3-glu on TNBC in the xenograft mouse model. Our data indicates that ... More
