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Custom Vector Construction> | … Each sample was repeated three times. The minimum detectable dose of MMP-19 was 0.01 ng/ml. Construction of recombinant vectors. Fragments encoding the full coding sequence of MMP-19, containing a flag insert following ATG, were synthesized by GenScript Co., Ltd … | Get A Quote |
The present study was designed to examine the expression and function of matrix metalloproteinase‑19 (MMP‑19), which is downregulated following respiratory syncytial virus (RSV) infection. The diverse expression levels of MMP were examined using a designed cDNA expression array. The expression and secretion of MMP‑19 was examined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis and ELISA, respectively. The proliferation of epithelial cells and lung fibroblasts were examined using flow cytometry. The epithelial‑mesenchymal transition (EMT) was also examined by performing western blot and RT‑qPCR analyses. The results of the cDNA assay showed that infection w... More