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Mechanism of super-assembly of respiratory complexes III and IV.

Nature. 2016-12; 
CogliatiSara,CalvoEnrique,LoureiroMarta,GuarasAdela M,Nieto-ArellanoRocio,Garcia-PoyatosCarolina,EzkurdiaIakes,MercaderNadia,VázquezJesús,EnriquezJosé Ant
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Custom Vector Construction … Molecular biology. The cDNA of SCAF1 113, 111 and the indicated mutants were synthesized and cloned in the pTNT vector by GenScript. Mitochondrial isolation. Mitochondria were isolated from mouse tissues as indicated elsewhere 12 . Mitochondrial import assay … Get A Quote

摘要

Respiratory chain complexes can super-assemble into quaternary structures called supercomplexes that optimize cellular metabolism. The interaction between complexes III (CIII) and IV (CIV) is modulated by supercomplex assembly factor 1 (SCAF1, also known as COX7A2L). The discovery of SCAF1 represented strong genetic evidence that supercomplexes exist in vivo. SCAF1 is present as a long isoform (113 amino acids) or a short isoform (111 amino acids) in different mouse strains. Only the long isoform can induce the super-assembly of CIII and CIV, but it is not clear whether SCAF1 is required for the formation of the respirasome (a supercomplex of CI, CIII and CIV). Here we show, by combining deep proteomics... More

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