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Novel STIM1-dependent control of Ca2+ clearance regulates NFAT activity during T-cell activation.

FASEB J.. 2016-12; 
SamakaiElsie,HooperRobert,MartinKayla A,ShmurakMaya,ZhangYi,KappesDietmar J,TemperaItalo,SoboloffJona
Products/Services Used Details Operation
Custom Vector Construction … The STIM1 luciferase vector was generated by insertion of a 780 bp sequence (−538 to +242 bp; synthesized by GenScript, Piscataway, NJ, USA) from the human STIM1 promoter into a firefly luciferase vector [hereafter referred to as STIM1 luciferase vector (S1-Luc)] … Get A Quote

摘要

Antigen presentation to the T-cell receptor leads to sustained cytosolic Ca elevation, which is critical for T-cell activation. We previously showed that in activated T cells, Ca clearance is inhibited by the endoplasmic reticulum Ca sensor stromal interacting molecule 1 (STIM1) via association with the plasma membrane Ca/ATPase 4 (PMCA4) Ca pump. Having further observed that expression of both proteins is increased in activated T cells, the current study focused on mechanisms regulating both up-regulation of STIM1 and PMCA4 and assessing how this up-regulation contributes to control of Ca clearance. Using a STIM1 promoter luciferase vector, we found that the zinc finger transcription factors early grow... More

关键词

EGR1,EGR4,Jurkat,PMCA4,SOCe,cal