至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The structure of a Trypanosoma cruzi glucose-6-phosphate dehydrogenase reveals differences from the mammalian enzyme.

FEBS Lett.. 2016-10; 
MercaldiGustavo F,DawsonAlice,HunterWillian N,CordeiroArt
Products/Services Used Details Operation
Custom Vector Construction … The synthetic gene was purchased (GenScript USA Inc., Piscataway, NJ, USA) and subcloned into a modified pET28 vector in which the sequence encoding a thrombin recognition site, to allow for removal of a N‐terminal histidine‐tag, was changed to that for tobacco etch virus … Get A Quote

摘要

The enzyme glucose-6-phosphate dehydrogenase from Trypanosoma cruzi (TcG6PDH) catalyses the first step of the pentose phosphate pathway (PPP) and is considered a promising target for the discovery of a new drug against Chagas diseases. In the present work, we describe the crystal structure of TcG6PDH obtained in a ternary complex with the substrate β-d-glucose-6-phosphate (G6P) and the reduced 'catalytic' cofactor NADPH, which reveals the molecular basis of substrate and cofactor recognition. A comparison with the homologous human protein sheds light on differences in the cofactor-binding site that might be explored towards the design of new NADP(+) competitive inhibitors targeting the parasite enzyme.

关键词

Chagas disease,drug discovery,ternary com