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A Novel Human scFv Library with Non-Combinatorial Synthetic CDR Diversity.

PLoS ONE. 2015; 
BaiXuelian,KimJihye,KangSeungmin,KimWankyu,ShimHy
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Custom Vector Construction … The template scFv genes (DP47-linker-DPK22 and DP47-linker-DPL3) were synthesized by GenScript Inc (Piscataway, NJ, USA) and cloned to pComb3X and pUC57 vectors The CDRs were amplified by PCR using specific primer sets (S1 Table) … Get A Quote

摘要

The present work describes the construction and validation of a human scFv library with a novel design approach to synthetic complementarity determining region (CDR) diversification. The advantage of synthetic antibody libraries includes the possibility of exerting fine control over factors like framework sequences, amino acid and codon usage, and CDR diversity. However, random combinatorial synthesis of oligonucleotides for CDR sequence diversity also produces many clones with unnatural sequences and/or undesirable modification motifs. To alleviate these issues, we designed and constructed a novel semi-synthetic human scFv library with non-combinatorial, pre-designed CDR diversity and a single native... More

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