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A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities

J Biol Eng.. 2019-07; 
Xu S,,, Wang F#,,, Wang Y,,, Wang R,,, Hou K,,, Tian C,,, Ji Y,,, Yang Q,,, Zhao P,,, Xia Q
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Molecular Biology Reagents The samples were analyzd on 4–20% SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) gels (Genscript, Nanjing, China) analyzed Get A Quote

摘要

BACKGROUND: Silk glands are used by silkworms to spin silk fibers for making their cocoons. These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention. RESULTS: In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system. The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34. Utilizing a simple purification protocol, 9.24 mg of the rhLF with reco... More

关键词

Glycosylation modification; High-efficiency production; Recombinant human lactoferrin; Silkworm