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Assembly and molecular architecture of the phosphoinositide 3-kinase p85α homodimer

J Biol Chem. 2015-12; 
LoPiccolo J, Kim SJ, Shi Y, Wu B, Wu H, Chait BT, Singer RH, Sali A, Brenowitz M, Bresnick AR, Backer JM.
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摘要

Phosphoinositide 3-kinases (PI3Ks) are a family of lipid kinases that are activated by growth factor and G-protein-coupled receptors and propagate intracellular signals for growth, survival, proliferation, and metabolism. p85α, a modular protein consisting of five domains, binds and inhibits the enzymatic activity of class IA PI3K catalytic subunits. Here, we describe the structural states of the p85α dimer, based on data from in vivo and in vitro solution characterization. Our in vitro assembly and structural analyses have been enabled by the creation of cysteine-free p85α that is functionally equivalent to native p85α. Analytical ultracentrifugation studies showed that p85α undergoes rapidly reversibl... More

关键词

analytical ultracentrifugation; molecular modeling; phosphatidylinositide 3-kinase (PI 3-kinase); phosphatidylinositol signaling; small-angle X-ray scattering (SAXS); structural model