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In Vitro Synthesis of Bioactive Glycovariants of Enterocin 96, an Antimicrobial Peptide from Enterococcus faecalis.

Methods Mol. Biol.. 2019; 
NagarRupa,Rao
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Gene Synthesis … Prepare all the solutions using molecular biology, proteomics, or mass spectrometry grade reagents as prerequisite … optimized bacterial glycosyltransferase gene (ORF 0417, Protein ID: WP_002382823.1 of Enterococcus faecalis TX0104, obtained from GenScript, USA, cloned … Get A Quote

摘要

Antimicrobial peptides (AMPs) are novel agents for therapeutic application for their inherent broad spectrum of activities against bacteria, fungi, and viruses, as well as anti-inflammatory, anticancerous, and immunomodulatory activities. This chapter presents an enzymatic method to generate glycovariants of one such antimicrobial peptide, namely enterocin 96, using a bacterial protein O- and S-glycosyltransferase, in vitro.

关键词

Chymotrypsin digestion,Exoglycosidase treatment/Deglycosylation assay,Glycodiversification,High performance liquid chromatography (HPLC),In vitro glycosyltransferase assay,Mass spectrom