Effects of Cdh23 single nucleotide substitutions on age-related hearing loss in C57BL/6 and 129S1/Sv mice and comparisons with congenic strains
| Products/Services Used |
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Operation |
| Gene Synthesis> |
To create the targeting vector, a synthetic gene (Genscript, Piscataway, NJ) consisting of a 300bp homology arm (Chr10: 60,536,591–60,536,889) and a 320 bp homology arm (Chr10: 60,527,162–60,527,481) and including restriction enzyme sites (HindIII and Xho1, spaced by Kpn1) for retrieval and Not1 for construct release was cloned into the pBlight-TK plasmid33 |
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