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Insertional mutagenesis identifies drivers of a novel oncogenic pathway in invasive lobular breast carcinoma

Nature Genetics. 2017;

Division of Molecular Pathology, The Netherlands Cancer Institute, Amsterdam, the Netherlands. Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Amsterdam, the Netherlands. Experimental Animal Pathology, The Netherlands Cancer Institute, Amsterdam, the Netherlands. Experimental Cancer Genetics, Wellcome Trust Sanger Institute, Hinxton, UK. Department of Pathology, The Netherlands Cancer Institute, Amsterdam, the Netherlands. Cancer Genomics Netherlands, The Netherlands Cancer Institute, Amsterdam, the Netherlands. Department of EEMCS, Delft University of Technology, Delft, the Netherlands.

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	The Ppp1r12aex1–9 sequence (NM_027892.2) was isolated from the cDNA of mouse tumor tissue using two subsequent Phusion Flash High-Fidelity (ThermoFisher Scientific) PCRs and subsequently cloned in a zero TOPO blunt vector (ThermoFisher Scientific). The Trp53bp2ex13–18 sequence (NM_ 173378.2) was commercially synthesized by Genscript	Get A Quote

摘要

Sjors M Kas, Julian R de Ruiter, Koen Schipper, Stefano Annunziato, Eva Schut1,Sjoerd Klarenbeek,Anne Paulien Drenth, Eline van der Burg, Christiaan Klijn, Jelle J ten Hoeve2、, David J，Adams，Marco J Koudijs, Jelle Wesseling, Micha Nethe, Lodewyk F A Wessels & Jos Jonkers