| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | pECUh6NSD3S (Enzimax, USA) and pECUh6PDP3 (Genscript, USA) 2 μ plasmids, containing URA3 as selectable marker and harboring the NSD3s and PDP3 genes, respectively, under control of the CUP1 promoter, were used to transform WT strain using the lithium acetate protocol [66]. Similarly, the Pdp3 W21A and the Pdp3 chimera carrying the PWWP domain of NSD3s, acquired from Genscript, USA, were expressed in Δpdp3 using the same vector. | Get A Quote |
WHSC1L1/NSD3， one of the most aggressive human oncogenes， has two isoforms derived from alternative splicing. Overexpression of long or short NSD3 is capable of transforming a healthy into a cancer cell. NSD3s， the short isoform， contains only a PWWP domain， a histone methyl-lysine reader involved in epigenetic regulation of gene expression. With the aim of understanding the NSD3s PWWP domain role in tumorigenesis， we used as an experimental model. We identified the yeast protein Pdp3 that contains a PWWP domain that closely resembles NSD3s PWWP. Our results indicate that the yeast protein Pdp3 and human NSD3s seem to play similar roles in energy metabolism， leading to a metabolic shift toward fe... More
