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Conformational Response of 30S-bound IF3 to A-Site Binders Streptomycin and Kanamycin.

Antibiotics (Basel). 2015; 
ChulluncuyRoberto,EspicheCarlos,NakamotoJose Alberto,FabbrettiAttilio,Milón
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Custom Vector Construction pET24c vectors containing wt and mutant genes were commercially acquired (GenScript, Piscataway, NJ, USA). Get A Quote

摘要

Aminoglycoside antibiotics are widely used to treat infectious diseases. Among them, streptomycin and kanamycin (and derivatives) are of importance to battle multidrug-resistant (MDR) . Both drugs bind the small ribosomal subunit (30S) and inhibit protein synthesis. Genetic, structural, and biochemical studies indicate that local and long-range conformational rearrangements of the 30S subunit account for this inhibition. Here, we use intramolecular FRET between the C- and N-terminus domains of the flexible IF3 to monitor real-time perturbations of their binding sites on the 30S platform. Steady and pre-steady state binding experiments show that both aminoglycosides bring IF3 domains apart, promoting a... More

关键词

30S subunit,FRET,IF3,kanamycin,streptomycin,translation initiation,tubercul