| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … 3. Experimental procedures. 3.1. Plasmid construction. The transfer vectors SINp-homing, SINp-source, SINp-detecting, SINp-null, and SINp-fusing were synthesized by Genscript (Pescataway, NY) and subcloned into the pUC57-Simple vector using EcoRV … | Get A Quote |
Challenges of cell-based cancer therapy include effectively homing therapeutic cells to the disease site and producing a controlled response in recognition of the microenvironment. While stem cells have been intensively studied for treating cancer as they naturally home towards sites of injury， they have drawbacks due to their pluripotency. In this paper we have shown that a stable HEK293 cell line expressing VEGFR2 and CarQ (an engineered Ca-sensitive RhoA) can home towards a cell colony expressing VEGF. Upon VEGF binding to VEGFR2， a Ca signal is produced that in turn activates CarQ-mediated cell blebbing. With the addition of VSVG， these homing cells can further initiate pH-dependent fusion upon reachi... More
